Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera.
J Clin Microbiol, 1977/1;5(1):1-4.
PMID: 188863
Impact factor: 11.677
Abstract
Neonatal calf diarrhea virus (a bovine rotavirus) formed distinct plaques in monolayers of MA-104 cells, an established macacus rhesus monkey kidney cell line, when diethylaminoethyl dextran and trypsin were included in the overlay medium. By using this plaque assay method, titration of neutralizing antibody to neonatal calf diarrhea virus was made feasible. It was demonstrated that some human sera contained neutralizing antibody to this agent.
MeSH terms
Animals; Animals, Newborn; Antibodies, Viral; Cattle; Child; Child, Preschool; Diarrhea Viruses, Bovine Viral; Humans; Infant; Neutralization Tests; RNA Viruses; Viral Plaque Assay
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