Preparation of plates with a permanent adsorbent layer and their application in the analytical thin-layer chromatography of lipids.
J Chromatogr, 1977/2/11;132(2):195-204.
Tsydendambaev VD, Zhukov AV, Vereshchagin AG
PMID: 188857
Abstract
Experimental conditions for preparing the thin-layer chromatographic plates with a permanent adsorbent layer (PAL) have been established; the particles of this layer are firmly bound to each other and to the glass support by means of fused glass powder. To prepare the PAL plates, a mixture of Woelm silica gel and glass powder (1:3, w/w) of particle size 7+/-1 mum was suspended in toluene and spread on the plates, yielding PALs of thickens 150-250 mum. The plates were heated in an electric furnace at 675 degrees for 20 min. In the separation of neutral lipids, a PAL thus prepared is equal in efficiency and selectivity to silica gel layers prepared in the usual manner.
MeSH terms
More resources
EndNote: Download