Resolution of DL-Phenylglycine by Penicillin G acylase.
Hindustan Antibiot Bull, 2005/2-2006/11;47-48(1-4):41-4.
Affiliations
PMID: 18697731
Abstract
The parameters for complete hydrolysis of L-phenyl acetyl phenylglycine (L-PAPG) using immobilized penicillin G acylase (IMEPGA) were investigated. IMEPGA exhibited maximum activity at pH 8.5 and 50 degrees C. The apparent Km value observed was 10 mM. Quantitative hydrolysis (>97%) of the L-PAPG was achieved within 45 min, at pH 7.8 and 37 degrees C, when 0.5% (w/v) of DL-PAPG was used and the concentration of IMEPGA was 133 IU/gm of DL-PAPG. The IMEPGA was used for 50 cycles.
MeSH terms
Enzymes, Immobilized; Glycine; Hydrogen-Ion Concentration; Penicillin Amidase; Polymers; Temperature
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