Analysis and purification of alcohol-sensitive chiral compounds using 2,2,2-trifluoroethanol as a modifier in supercritical fluid chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci, 2008/11/01;875(1):237-42.
Byrne N[1], Hayes-Larson E, Liao WW, Kraml CM
Affiliations
PMID: 18676209DOI: 10.1016/j.jchromb.2008.06.057
Impact factor: 3.318
Abstract
2,2,2-Trifluoroethanol (TFE) is evaluated as an alternative modifier for the analysis and purification of alcohol-sensitive chiral compounds using supercritical fluid chromatography (SFC). Four chiral compounds, selected for their sensitivity to alcohols, in addition to a variety of standard chiral compounds were analyzed by SFC using TFE with polysaccharide and Pirkle-type chiral stationary phases (CSPs) to produce selectivities (alpha) and resolutions (Rs) as high as 1.4 and 7.2. A preparative isolation of 2-phenylglutaric anhydride was achieved using TFE as the mobile phase modifier to produce clean enantiomers.
MeSH terms
Anhydrides; Chromatography, Supercritical Fluid; Glutarates; Pharmaceutical Preparations; Stereoisomerism; Trifluoroethanol
More resources
EndNote: Download