Phosphorylation of synaptic-membrane proteins from ox cerebral cortex in vitro. Partition of substrates and protein kinase activities with triton X-100.
Biochem J, 1976/9/01;157(3):661-6.
Dunkley PR, Holmes H, Rodnight R
PMID: 186029
Impact factor: 3.766
Abstract
Synaptic-membrane fragments from ox cerebral cortex contain basal and cyclic AMP-stimulated protein kinase activity catalysing the phosphorylation of endogenous substrates. Extraction of membrane fragments with Triton X-100 solubilized less than 20% of the kinase activity and left the major part of the endogenous substrates in the insoluble fraction.
MeSH terms
Animals; Cattle; Cerebral Cortex; Cyclic AMP; Male; Nerve Tissue Proteins; Phosphoproteins; Protein Kinases; Synaptic Membranes
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