Partial purification and characterization of the mRNA for rat preproinsulin.
Proc Natl Acad Sci U S A, 1976/10;73(10):3539-43.
Duguid JR, Steiner DF, Chick WL
PMID: 185618
Impact factor: 12.779
Abstract
Electrophoretically homogeneous messenger RNA for rat preproinsulin has been prepared from an islet cell tumor by the use of oligo(dT(-cellulose chromatography and sucrose density gradient centrifugation. The molecular weight of the mRNA is about 210,000, as determined by polyacrylamide gel electrophoresis in formamide, and its sedimentation coefficient is 9.3 S in sucrose gradients containing 0.2 M NaCl. These results indicate that the synthesis of preproinsulin is directed by a monocistronic mRNA 600 nucleotides in length and requires approximately 55% of the molecule's coding capacity.
MeSH terms
Adenoma, Islet Cell; Animals; Centrifugation, Density Gradient; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Molecular Weight; Neoplasms, Experimental; Proinsulin; Protein Precursors; RNA, Messenger; Rats
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