Practical considerations for using mag-fura-2 to measure cytosolic free magnesium.
Magnes Trace Elem, 1991-1992;10(2-4):142-50.
Grubbs RD[1], Beltz PA, Koss KL
Affiliations
PMID: 1844547
Abstract
The development of fluorescent ion-selective indicators for magnesium has provided valuable tools for measuring second-by-second changes in cytosolic magnesium activity. In the course of establishing appropriate protocols for using one of these indicators, mag-fura-2, to measure magnesium activity in BC3H-1 cells and chick ventricular myocytes, many potential pitfalls and limitations of this technique have been encountered and addressed. These observations are presented for the purpose of providing guidelines regarding the appropriate use of this indicator and on factors influencing the interpretation of resulting data.
MeSH terms
Animals; Calcium; Cells, Cultured; Chick Embryo; Energy Metabolism; Fluorescent Dyes; Fura-2; Heart Ventricles; Magnesium; Mice; Muscle, Smooth
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