Effect of metabolites on epsilon-N-hydroxylysine formation in cell-free extracts of Aerobacter aerogenes 62-1.
Can J Biochem, 1977/6;55(6):625-9.
Murray GJ, Clark GE, Parniak MA, Viswanatha T
PMID: 18266
Abstract
The conversion of L-lysine to its corresponding epsilon-N-hydroxy derivative has been achieved for the first time by cell-free extracts of Aerobacter aerogenes 62-1. Partial fractionation by differential centrifugation (at 12 000 X g) revealed that both supernatant and pellet are essential for maximum enzymatic activity. The omega-N-hydroxylase (EC 1.14.99) was found to function optimally at pH 7-7.5 and exhibited an apparent Km of about 75 muM for L-lysine. L(+)-Lactate or DL-lactate and pyruvate greatly stimulate the omega-N-hydroxylase activity. The system is strongly inhibited by arsenite and sulfite.
MeSH terms
Arsenic; Cell-Free System; Enterobacter; Enterobacteriaceae; Hydrogen-Ion Concentration; Hydroxylysine; Lactates; Lysine; Mixed Function Oxygenases; Pyruvates; Structure-Activity Relationship; Subcellular Fractions; Sulfites
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