Microtiter assay for interferon: microspectrophotometric quantitation of cytopathic effect.
Appl Environ Microbiol, 1976/1;31(1):35-8.
PMID: 182074
Impact factor: 5.005
Abstract
This report describes an accurate, reproducible, and efficient microassay for human interferon, using a dye-uptake method to quantitate cytopathogenicity. The antiviral activity was measured by using a Gilford 300-N microsepctrophotometer, with the automatic programmer, sampler, and data lister. Two hundred interferon samples, each in a final volume of 1.0 ml, may be analyzed and recorded in 45 min. The reproducibility of a laboratory reference interferon and the human international reference B 69/19 on two different cell lines, using the model Q Oxford sampler, was found to be excellent, with the standard error of the log10 geometric mean of both references +/- 0.04 to 0.06.
MeSH terms
Biological Assay; Cell Line; Cytopathogenic Effect, Viral; Evaluation Studies as Topic; Fibroblasts; Humans; Interferons; Neutral Red; Spectrophotometry; Vesicular stomatitis Indiana virus
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