Probing the distance between the two binding sites of hirudin for its interaction with the active site and the fibrin(ogen)-binding site of alpha-thrombin.
Biomed Biochim Acta, 1991;50(4-6):707-10.
Wagner U[1], Schmitz T, Otte M, Dodt J
Affiliations
PMID: 1801747
Abstract
The interaction of delta (Ser50)-hirudin with alpha-thrombin has been investigated. Deletion of Ser50 of r-hirudin caused a 2.7 fold increase of the Ki for its complex with alpha-thrombin. Determination of the rate constants kon and koff for complex formation showed that this effect was mainly due to a change in koff.
MeSH terms
Amino Acid Sequence; Animals; Binding Sites; Fibrin; Fibrinogen; Hirudins; Humans; Kinetics; Molecular Sequence Data; Mutation; Oligopeptides; Thrombin
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