Isolation and characterization of serologically active phosphatidylinositol oligomannosides of Mycobacterium tuberculosis.
J Biochem, 1975/9;78(3):547-54.
PMID: 178641
Impact factor: 3.241
Abstract
Phospholipids extracted with hot methanol from Mycobacterium tuberculosis, strain H37Rv, were fractionated with solvent mixtures and by partition chromatography on silica-gel columns. Of 12 components detected on a thin-layer chromatogram of the total phospholipid fraction, 6 components were purified. They were all phosphatidylinositol oligomannosides: two hexamannosides (H-2 and H-3), one tetramannoside (H-4), one trimannoside (H-5), and two dimannosides (H-6 and H-7). The molar ratios of fatty acid to phosphorus were 3 for H-2, H-4, H-5, and H-6, and 4 for H-3 and H-7. In each lipid, more than 80% of the total fatty acids were palmitic and tuberculostearic acids. Each of the six lipids had serological activity to various degrees.
MeSH terms
Chromatography, Gas; Chromatography, Gel; Chromatography, Thin Layer; Fatty Acids; Hemagglutination Tests; Humans; Mannose; Mycobacterium tuberculosis; Oligosaccharides; Phosphatidylinositols; Phospholipids
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