Evidence for a one-hit theory in the immune bactericidal reaction and demonstration of a multi-hit response for hemolysis by streptolysin O and Clostridium perfringens theta-toxin.
Infect Immun, 1976/2;13(2):337-44.
Inoue K, Akiyama Y, Kinoshita T, Higashi Y, Amano T
PMID: 177364
Impact factor: 3.609
Abstract
An analytical method was developed for estimating the number of hits necessary to lyse or kill cells in which various concentrations of the cells are treated with a constant amount of the lytic or killing agent in a constant reaction volume. The reaction may be due to a single-component agent or occur by a sequential chain of reactions due to a multi-component agent, even including side, abortive, or counter-reactions. It was clearly shown by this method that immune bactericidal reactions followed a one-hit theory. It was shown by this method that streptolysin O required four or five hits for hemolysis and Clostridium perfringens theta-toxin required two hits. These results were confirmed by both logarithmic dose-response and survival analyses. It was also shown that streptolysin O and theta-toxin can act complementarily on accumulation of the hits for hemolysis.
MeSH terms
Animals; Blood Bactericidal Activity; Clostridium perfringens; Hemolysis; In Vitro Techniques; Models, Biological; Streptolysins; Toxins, Biological
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