Synthesis of 2-n-(hexadecanoyl)-amino-4-nitrophenyl phosphorylcholine-hydroxide, a chromogenic substrate for assaying sphingomyelinase activity.
Chem Phys Lipids, 1976/2;16(1):71-9.
PMID: 177220
Impact factor: 3.57
Abstract
2-N-(Hexadecanoyl)-amino-4-nitrophenyl phosphorylcholine-hydroxide a compound resembling sphingomyelin is synthesized. It is cleaved by sphingomyelinase to the chromogenic N-acylaminonitrophenyl moiety. Phospholipase C preparations do not hydrolyze this compound. The starting material is 2-amino-4-nitrophenol which when acylated with palmitoyl chloride yields the hexadecananilide. Reaction with beta-bromoethylphosphoryldichloride gives the phosphate which is quaternized with trimethylamine to give the title compound.
MeSH terms
Magnetic Resonance Spectroscopy; Methods; Molecular Conformation; Phospholipids; Phosphoric Diester Hydrolases; Spectrophotometry, Infrared; Sphingomyelin Phosphodiesterase
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