Kinetics of utilization of Sendai virus RNA and protein in the process of virion assembly.
J Virol, 1976/2;17(2):605-13.
PMID: 176433
Impact factor: 6.549
Abstract
The synthesis of the 50S genomic RNA and strucural proteins of Sendai virus was examined with respect to their utilization in virus assembly. It was found that during a single cycle of infection, 50S RNA was synthesized before the structural proteins and that both RNA and protein were synthesized 2 to 4 h before their appearance in released virions. Pulse-chase labeling indicated that the NP and P proteins synthesized early and the M and F proteins synthesized late were preferentially incorporated into virus relative to the other viral proteins. The kinetics of incorporation of pulse-labeled NP protein suggested that it was withdrawn from a relatively large pool whereas the M protein appeared to be present in a relatively small pool in the cytoplasm. Further, it was possible to chase pulse-labeled M protein, but not NP protein, from the cell during an 8-h time period.
MeSH terms
Animals; Cattle; Cell Line; Morphogenesis; Parainfluenza Virus 1, Human; RNA, Viral; Virus Replication
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