Mitogen induction of murine C-type viruses. I. Analysis of lymphoid cell subpopulations.
J Immunol, 1976/4;116(4):1145-50.
PMID: 176274
Impact factor: 5.426
Abstract
We reported previously in vitro induction of endogenous C-type viruses from normal mouse spleen cells by lipopolysaccharide (LPS) as well as by combination treatment with concanabalin A and 5-bromo-2'-deoxyuridine (Con A/BrdU). To identify the cell types sensitive to virus induction and to study the relationship of mitogenicity to virus induction we have compared T cell populations (BALB/c thymus cells and cortisone-resistant thymus cells), B cell populations (nu/nu spleen cells and lymph node cells), adherent BALB/c peritoneal cells and mixed populations (BALB/c spleen cells, macrophage-depleted BALB/c spleen cells, and lymph node cells). LPS-induction occurred only in B cell-containing populations. In contrast, induction by Con A/BrdU depended on the presence of both T and B cells. In both instances, neither macrophages nor hemopoietic cells appeared to be a major source of virus. Treatment with anti-Ig serum and complement reduced virus induction by LPS/BrdU but not by Con A/BrdU suggesting that different cell populations produce virus after stimulation with these two different mitogens.
MeSH terms
Animals; Antibodies, Anti-Idiotypic; B-Lymphocytes; Complement System Proteins; Concanavalin A; Lipopolysaccharides; Lymph Nodes; Macrophages; Mice; Mice, Inbred BALB C; Mice, Nude; Mitogens; Retroviridae; Spleen; T-Lymphocytes
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