Growth characteristics of bovine herpesvirus 1 (infectious bovine rhinotracheitis) in human diploid cell strain WI-38.
Proc Soc Exp Biol Med, 1976/2;151(2):407-10.
Michalski FJ, Dietz A, Hsiung GD
PMID: 175382
Abstract
IBR virus was found to replicate in WI-38 cells. At a high input multiplicity the virus yield was comparable to that obtained in bovine cells, but comparable degree of CPE took longer to achieve. At a low input multiplicity of IBR virus, such as may be encountered in virus contaminated bovine serum, virus yield was only about 1% of that in bovine cells, with 50% of the cells showing CPE, followed by cell regrowth. Infectious virus was not recoverable from the regrown cells by 5 weeks after initial infection, and these regrown cells were susceptible to reinfection with IBR virus. Aging of WI-38 cells in cultures for as little as 1 week reduced IBR virus yield to 90% less than the yield from the same lot of cells inoculated 7 days earlier.
MeSH terms
Age Factors; Cells, Cultured; Cytopathogenic Effect, Viral; Herpesvirus 1, Bovine; Humans; Species Specificity; Virus Replication
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