[A micromethod for determination of the adenylate cyclase activity by chromatography on silufol UV-254 plates].
Vopr Med Khim, 1975/5-1975/6;21(3):322-4.
PMID: 174304
Abstract
A method is developed for micro-scale separation of the products of enzymatic degradation of ATP (ADP, 5'-AMP, adenosine 3',5'-cytophosphate, adenosine and adenine) by one-dimentional thin-layer chromatography on Silufol UV-254 plates. The method is applicable for determination of activity of adenylate cyclase and of other enzymes involved in metabolism of adenylic derivatives, including the diesterase of 3',5'-cyclic AMP.
MeSH terms
3',5'-Cyclic-AMP Phosphodiesterases; Adenylyl Cyclases; Chromatography, Thin Layer; Microchemistry; Ultraviolet Rays
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