Adaptation of N-tropic Friend leukaemia virus and its murine sarcoma virus pseudotype of non-permissive B-type C57BL/6 mouse cell line.
J Gen Virol, 1975/10;29(1):1-9.
PMID: 171332
Impact factor: 5.141
Abstract
N-tropic Friend leukaemia virus (FLV) was serially passaged in a B-type C57BL/6 mouse cell line, YH-7. After a single passage, the infectious efficiency of FLV in the restrictive YH-7 cells was significantly increased. This adapted character of FLV could be reversed by a single passage in permissive N-type MLg cells. The true host range conversion from N to NB was accomplished after eleven to twelve passages in YH-7 cells. In contrast, the host range conversion of N-tropic murine sarcoma pseudotype, MuSV(FLV), took place after two passages.
MeSH terms
Adaptation, Biological; Animals; Cell Line; Friend murine leukemia virus; Gammaretrovirus; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Sarcoma Viruses, Murine; Species Specificity; Virus Replication
More resources
EndNote: Download