Intracellular synthesis of mouse mammary tumor virus polypeptides: indication of a precursor glycoprotein.
J Virol, 1975/8;16(2):250-8.
PMID: 168401
Impact factor: 6.549
Abstract
Mouse mammary tumor virus polypeptides were detected in the cytoplasm of mouse mammary tumor cell cultures using immunological precipitation techniques. The anti-mouse mammary tumor virus serum precipitated the major virion glycoproteins gp49 and gp37.5/33.5 and a viral-related nonvirion glycoprotein of 76,000 daltons. Subcellular fractionation studies revelaed that the cell-associated virion glycoproteins were present in the membrane fraction. Pulsechase experiments indicated that a viral-related nonvirion glycoprotein of 76,000 daltons may be a precursor to one or more of the virion glycoproteins.
MeSH terms
Adenocarcinoma; Animals; Cell Fractionation; Cell Membrane; Cell-Free System; Culture Techniques; Cytoplasm; Electrophoresis, Polyacrylamide Gel; Glucosamine; Glycoproteins; Mammary Neoplasms, Experimental; Mammary Tumor Virus, Mouse; Methionine; Mice; Molecular Weight; Neoplasm Proteins; Peptide Biosynthesis; Precipitin Tests; Protein Precursors; Sulfur Radioisotopes; Tritium; Viral Proteins
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