The ultraviolet endonuclease of bacteriophage T4. Further characterization.
J Biol Chem, 1975/4/25;250(8):2823-9.
Minton K, Durphy M, Taylor R, Friedberg EC
PMID: 164454
Abstract
The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.
MeSH terms
Animals; Cattle; Coliphages; DNA Viruses; DNA, Single-Stranded; DNA, Viral; Deoxyribonucleases; Endonucleases; Kinetics; Molecular Weight; Nucleic Acid Denaturation; Phosphoric Diester Hydrolases; Radiation Effects; Spleen; Thymine; Time Factors; Ultraviolet Rays
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