Modification of nuclear restriction in vitro by plasma from tumor-bearing animals.
J Natl Cancer Inst, 1975/1;54(1):123-8.
PMID: 163310
Impact factor: 11.816
Abstract
Putative regulatory macromolecules, which may be useful in diagnosis or tumor detection, were identified in the peripheral blood plasma of tumor-bearing animals. We monitored the components by measuring their ability to stimulate messenger RNA (mRNA) release from isolated nuclei in a cell-free system of rat liver nuclei in fortified homologous cytosol. This in vitro test system exhibited near-normal in vivo nuclear RNA restriction. When added to the assay at a protein concentration of 3.0 mg/ml, dialyzed plasma from rats or mice with chemically induced transplantable or primary tumors stimulated mRNA release from 87% to more than 300% over control plasma from normal rats. Plasma from partially hepatectomized rats stimulated only 26% over control plasma. The test system derived from rat liver seemed to permit the monitoring of plasma from other species. Available evidence, particularly relating to tumor-host-interaction, suggests, but does not prove, that regulatory components are released from the tumor cells to the circulation.
MeSH terms
Animals; Base Sequence; Blood Proteins; Carcinoma, Hepatocellular; Cell Nucleus; Cell-Free System; Cytosol; Female; Hepatectomy; Liver; Liver Neoplasms; Liver Regeneration; Macromolecular Substances; Male; Mammary Neoplasms, Experimental; Neoplasms; Neoplasms, Experimental; Nucleic Acid Hybridization; Plasma; RNA, Messenger; Rats; Sarcoma, Experimental
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