Characteristics of mammary tumor cultures from four mouse strains infected with mammary tumor virus.
Cancer Res, 1975/2;35(2):370-3.
PMID: 162873
Impact factor: 13.312
Abstract
The morphology and production of oncornaviruses in primary cell and explant mammary adenocarcinoma cultures derived from C3Hf, BALB/cNIV, BALB/cfC3H, and C3H mice were characterized. Cultures from the four mouse strains were morphologically similar; they were all epithelial and formed hemicysts and mounds. However, the numbers of cells containing mammary tumor virus (MTV) antigens and the production of cell-free MTV varied greatly among the established cultures. The percentages of MTV-positive cells in the cultures correlated with detection of cell-free MRV; immunodiffusion assays of virions from C3Hf and BALB/cNIV cultures were consistently negative for MTV antigens, whereas virions from BALB/cfC3H and C3H cultures were always positive. Synthesis of murine leukemia virus group-specific, soluble antigens was always observed in C3Hf, BALB/cNIV, and C3H cultures but only rarely observed in BALB/cfC3H cultures.
MeSH terms
Adenocarcinoma; Animals; Antigens, Viral; Cells, Cultured; Fluorescent Antibody Technique; Genotype; Immunodiffusion; Mammary Neoplasms, Experimental; Mammary Tumor Virus, Mouse; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred Strains
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