Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly.
Bioelectrochemistry, 2005/4;66(1-2):139-43.
Calvo-Muñoz ML[1], Dupont-Filliard A, Billon M, Guillerez S, Bidan G, Marquette C, Blum L
Affiliations
PMID: 15833714
Impact factor: 5.76
Abstract
The electrochemiluminescence (ECL) of a luminol derivate (ABEI) generated both by a carbon electrode and a polypyrrole-coated carbon electrode was examined. It was found that the polypyrrole film (ppy) did not inhibit the ECL. After that, ABEI anchored on a single stranded DNA target (ODNt) has been used for the ECL detection of the hybridization between a complementary single stranded DNA probe (ODNp) covalently linked to a polypyrrole support and the ODNt. The ECL detection has been performed using a DNA sensor having a low surface concentration of ODNp probes, constituted of a polypyrrole copolymer electrosynthesized from a pyrrole-ODNp/pyrrole monomer ratio of 1/20,000.
MeSH terms
Electrochemistry; Electrodes; Luminescent Measurements; Luminol; Membranes, Artificial; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Polymers; Pyrroles
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