In vitro immune response of human peripheral lymphocytes. II. Properties and functions of concanavalin A-induced suppressor T cells.
Microbiol Immunol, 1979;23(3):185-96.
Kuritani T, Hirano T, Kishimoto T, Yamamura Y
PMID: 158124
Impact factor: 2.962
Abstract
Con A-stimulation of human peripheral T lymphocytes induced both suppressor and helper T cells. ConA-generated suppressor T cells inhibited PWM-induced IgG and IgM production in PBL. Lower concentrations of Con A (0.5 micrograms/ml) or shorter incubation periods (6 to 24 hr) induced mainly helper T cells, while higher concentrations of Con A (10 micrograms/ml) or longer incubation periods (at least 48 hr) induced suppressor T cells. Con A-generated suppressor T cells were sensitive to mitomycin treatment and exerted their suppressor function on the early phase of differentiation and/or proliferation of B cells but not on the final differentiation of B cells to Ig-producing cells. The identity of the MHC was not required for the expression of suppressor function. Suppressor T cells competed with helper T cells in PWM-induced Ig-production of PBL. This experimental system can be applied to estimate the regulatory function of T cells in several disease states.
MeSH terms
B-Lymphocytes; Concanavalin A; Dose-Response Relationship, Immunologic; Humans; Immunoglobulin G; Immunoglobulin M; Immunoglobulins; Mitomycins; T-Lymphocytes; T-Lymphocytes, Regulatory
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