[3H-thymidine-paper strip-counting of in vitro lymphocyte transformation. II. Specific antigens in the whole blood assay (author's transl)].
Allerg Immunol (Leipz), 1979;25(1):54-70.
Schütt C, Göcke H, Schulze HA, Westphal HJ, Glawe HJ
PMID: 157683
Abstract
Erythrocytes did not influence the harvesting procedure or the paper strip counting method in a liquid scintillation counter using the whole blood assay of lymphocyte transformation test. The leucocyte-erythrocyte-ratio influences the blastogenesis in vitro. Only 1--2 ml of peripheral blood are necessary to proof lymphocyte reactivity to mitogens or antigens. The storage of the blood is possible over a period of 24 hours (4 degrees C) if the absolute lymphocytes count remains constant.
MeSH terms
Adolescent; Adult; Aged; Antigens, Fungal; Antigens, Viral; Candida albicans; Child; Child, Preschool; Erythrocytes; Humans; Infant; Lymphocyte Activation; Middle Aged; Mitogens; Mumps virus; Paper; Thymidine; Tuberculin
More resources
EndNote: Download