Differentiation of mouse myeloid leukemia cells is inhibited by a factor from non-differentiating leukemia cells.
Int J Cancer, 1978/11/15;22(5):570-5.
Okabe J, Hayashi M, Honma Y, Hozumi M
PMID: 152739
Impact factor: 7.316
Abstract
Mouse myeloid leukemia cells (MI) were induced to differentiate by a factor(s) (D-factor) in ascitic fluid. An inhibitory activity (I-activity) for the induction of differentiation was present in conditioned medium and lysate of MI cells resistant to the D-factor. The I-activity was non-dialyzable, heat-labile and protease-sensitive. Most of the activity was recovered in the fraction precipitated with 30-50% saturated ammonium sulfate. The fraction inhibited induction of phagocytic activity, migrating activity and morphological changes in MI cells, which are typical properties of differentiated MI cells. Low levels of I-activity were detected in conditioned medium or lysate of MI cells sensitive to the D-factor. The resistant MI cells were sensitized to the D-factor by treatment with a low concentration (5-10 ng/ml) of actinomycin D. The I-activity in conditioned medium of actinomycin D-treated resistant cells decreased with development of sensitivity to the D-factor. These results suggest that production of the I-activity in the resistant cells is closely associated with resistance of the MI cells to the D-factor.
MeSH terms
Animals; Ascitic Fluid; Cell Line; Clone Cells; Culture Media; Dactinomycin; Drug Resistance; Floxuridine; Growth Inhibitors; Hematopoiesis; Leukemia, Experimental; Leukemia, Myeloid; Leukocytes; Mice; Peptide Hydrolases; Phagocytosis; Rats
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