In vivo transfer of the ti-plasmid of Agrobacterium tumefaciens to Escherichia coli.
Mol Gen Genet, 1978/7/25;163(3):335-8.
Holsters M, Silva B, Van Vliet F, Hernalsteens JP, Genetello C, Van Montagu M, Schell J
PMID: 150536
Abstract
The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transfer and maintain themselves very efficiently in E. coli. An E. coli harbouring such a Ti::RP4 plasmid is unable to catabolize octopine and unable to induce crown-gall tumours on plants.
MeSH terms
Conjugation, Genetic; Drug Resistance, Microbial; Escherichia coli; Phenotype; Plasmids; Rhizobium; Rifampin; Spectinomycin; Streptomycin; Transformation, Bacterial
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