[Perfusion of isolated rat liver. Utilization of a perfusion without heparin. Validity of the preparation].
C R Seances Soc Biol Fil, 1977;171(3):508-12.
Trupin N, Nguyen M, Louf B, Dewailly P, Vanlerenberghe J
PMID: 143984
Abstract
The blood and liver giver animals are given "Tromexan" for a period of three days. The blood defibrination is finished off by shaking. The perfusion is made of defibrinated blood to which is added a compounding of mineral salts, albumin and glucose in adequate proportions. The fluid is used during 150 minutes with a biliary flow of 4 ul/minute (instead of 8 in perfusions with heparine). The validity of the preparation is tested by biliary acids, cholesterol and Brom Sulfon Phtalein biliary elimination research. This fluid can be used when heparin is avoided: for example in a case of investigation of fat emulsion scrubbing.
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