[Interaction of a membrane preparation of Na+, K+-ATPase with a spin-labeled analog of ATP].
Biokhimiia, 1977/3;42(3):476-80.
Tabak M, Ruuge EK, Smirnova IN, Petrov AI, Sukhorukov BI
PMID: 140708
Abstract
Interaction of membrane Na+, K+-ATPase preparation from brain gray matter with spin-labelled ATP analogue, in which free iminoxyl radical is joined as a result of 2'(3')-OH ribose groups acylation, is studied. The rotatory mobility of spin-labelled ATP analogue in Na+,K+-ATPase preparation is found to change in non-linear manner during temperature variation (the break-point on the curve being at 20-23degrees C). It correlates with temperature dependence of Na+,K+-ATPase and temperature dependence of lipid viscosity in the membranes, determined by means of hydrophobic spin probes. Substitution of Mg2+ ions with paramagnetic Mn2+ ions resulted in an intense magnetic dipole-dipole interaction between a spin label and Mn2+ ion, which indicated the formation of triple complex enzyme--spin-labelled ATP--Mn2+.
MeSH terms
Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Brain; Catalysis; Cattle; Chemical Phenomena; Chemistry; Manganese; Membranes; Spin Labels; Temperature
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