Energy coupling and respiration in Nitrosomonas europaea.
Arch Microbiol, 1976/11/02;110(23):257-62.
PMID: 13754
Impact factor: 2.667
Abstract
Intact cells of Nitrosomonas europaea grown in an ammonium salts medium will oxidise ammonium ions, hydroxylamine and ascorbate-TMPD; there is no oxidation of carbon monoxide, methane or methanol. The Km value for ammonia oxidation is highly pH dependent with a minimum value of 0.5 mM above pH 8.0. This suggests that free ammonia is the species crossing the cytoplasmic membrane(s). The measurement of respiration driven proton translocation indicates that there is probably only one proton translocating loop (loop 3) association with hydroxylamine oxidation. The oxidation of "endogenous" substrates is sometimes associated with more than one proton-translocating loop. These results indicate that during growth hydroxylamine oxidation is probably associated with a maximum P/O ratio of 1.
MeSH terms
Electron Transport; Hydrogen-Ion Concentration; Hydroxylamines; Nitrosomonas; Oxidation-Reduction; Oxidative Phosphorylation; Quaternary Ammonium Compounds
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