Glycosaminoglycans and proteoglycans of human chondrosarcoma.
Biochim Biophys Acta, 1976/6/23;437(1):71-86.
Sweet MB, Thonar EM, Immelman AR
PMID: 132969
Abstract
The glycosaminoglycans and proteoglycans of a human chondrosarcoma have been studied. Glycosaminoglycans were fractionated and identified by cetylpirdium chloride (CPC) cellulose chromatography, ECTEOLA cellulose ion-exchange chromatography and electrophoresis on cellulose acetate. Proteoglycans were extracted by low ionic strength solutions and by 4 M guanidinium chloride and fractioned by equilibrium density-gradient centrifugation and gel chromatography on Sepharose 2B. The tumour matrix contained both the 4- and 6-sulphate isomers of chondroitin sulphate and a high concentration (12% of hexosamine) of hyaluronic acid. Proteoglycans were poor in carbohydrate moieties and proportion were capable of aggregation. Amino acid analysis of the fractionated proteoglycans suggested the presence of a single protein core. A substance with the characteristic amino acid composition of glycoprotein link was recovered from the top of the dissociative density gradient.
MeSH terms
Amino Acids; Chondrosarcoma; Galactosamine; Glucosamine; Glycosaminoglycans; Humans; Male; Middle Aged; Papain; Proteoglycans; Spectrophotometry, Infrared; Uronic Acids
More resources
EndNote: Download