[Fluorescent antibody method in the study of an antiviral agent: 5-sulfamido--isatin-thiosemicarbazone].
Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol, 1975/4-1975/6;20(2):113-8.
Alexandrescu M, Handrache L, Murgescu C
PMID: 128095
Abstract
Direct immunofluorescence supplied evidence of the antiviral action of a new synthetic derivative (5-sulfamido-ITSC) against vaccina virus in HeLa cell cultures. The antiviral potential of the new derivative is expressed by a 10-18 delay in the appearance of the viral antigen and by the quantitative reduction of synthesis. Administered in a single dose of 40 uM/ml, after infection of the HeLa cells, 5-sulfamido-ITSC does not arrest the infection and bring about elimination of the virus from the cell, in the course of a single viral multiplication cycle. Direct immunofluorescence is a rapid, specific method for detection of the in vitro antiviral action of synthetic derivatives.
MeSH terms
Antiviral Agents; Fluorescent Antibody Technique; HeLa Cells; Indoles; Isatin; Sulfonamides; Thiosemicarbazones; Vaccinia virus; Virus Replication
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