Establishment of an R-banded rabbit karyotype nomenclature by FISH localization of 23 chromosome-specific genes on both G- and R-banded chromosomes.
Cytogenet Genome Res, 2002;98(2-3):199-205.
Hayes H[1], Rogel-Gaillard C, Zijlstra C, De Haan NA, Urien C, Bourgeaux N, Bertaud M, Bosma AA
Affiliations
PMID: 12698004
Impact factor: 1.941
Abstract
Direct detection of fluorescent in situ hybridization signals on R-banded chromosomes stained with propidium iodide is a rapid and efficient method for constructing cytogenetic maps for species with R-banded standard karyotypes. In this paper, our aim is to establish an R-banded rabbit karyotype nomenclature that is in total agreement with the 1981 G-banded standard nomenclature. For this purpose, we have produced new GTG- and RBG-banded mid-metaphase karyotypes and an updated version of ideograms of R-banded rabbit chromosomes. In addition, to confirm correlations between G- and R-banded chromosomes, we have defined a set of 23 rabbit BAC clones, each containing a specific gene, one marker gene per rabbit chromosome, and we have localized precisely each BAC clone by FISH on both G- and R-banded chromosomes.
MeSH terms
Animals; Chromosome Banding; Chromosome Mapping; Chromosomes, Mammalian; Genes; Genetic Markers; In Situ Hybridization, Fluorescence; Karyotyping; Rabbits; Terminology as Topic
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