Microheterogeneity of chondroitin sulfates from various cartilages.
Connect Tissue Res, 1975;3(1):87-96.
Seno N, Anno K, Yaegashi Y, Okuyama T
PMID: 126131
Impact factor: 3.342
Abstract
Chondroitin sulfate fractions were isolated from different animal cartilages, including whale, cattle, sheep, ray and shark, by Dowex 1 chromatography followed by ethanol fractionation. Although each preparation showed a single spot when electrophoresed on cellulose acetate, both 4- and 6-sulfated disaccharides were present in chondroitinase digests of each. In particular, the main fraction of bovine tracheal chondroitin sulfate (SO4/Ga1N = 1) gave both the disaccharides in nearly equal amounts, and its IR spectrum showed absorption bands at 820 and 850 cm-1. This fraction yielded three types of tetrasaccharides after digestion with testicular hyaluronidase. Structural studies on these tetrasaccharides, using P. vulgaris chondro-4-sulfatase followed by chondroitinase, showed that one of them is a hybrid consisting of the 4- and 6-sulfated residues. In the light of these facts, a nomenclature for chondroitin sulfates is discussed.
MeSH terms
Animals; Cartilage; Cattle; Chondroitin; Chondroitin Sulfates; Disaccharides; Fishes; Nose; Sharks; Sheep; Skull; Terminology as Topic; Trachea; Uronic Acids; Whales
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