Measurement of urinary mucopolysaccharides.
S Afr Med J, 1975/6/21;49(26):1038-40.
PMID: 125458
Impact factor: 2.162
Abstract
The fact that the polyanionic long chains of mucopolysaccharides (MPS) can be precipitated with detergents such as cetylpiridiniumchloride (CPC) has been used in the development of a method for the quantitative estimation of total MPS in urine. Urine samples are all brought to an osmolarity of 300, after which an equal volume of 0,1% CPC is added to each sample. The turbidity is determined spectrophotometrically after 5 minutes. Chondroitin sulphate A was used as an internal standard. Normal values obtained for different age groups in the population of Pretoria are discussed.
MeSH terms
Adolescent; Adult; Age Factors; Cetylpyridinium; Chemistry, Clinical; Child; Child, Preschool; Creatinine; Female; Glycosaminoglycans; Humans; Male; Nephelometry and Turbidimetry; Osmolar Concentration; Urine
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