A radiometric microbiologic assay for the biologically active forms at niacin.
J Nucl Med, 1979/5;20(5):419-23.
Kertcher JA, Guilarte TR, Chen MF, Rider AA, McIntyre PA
PMID: 120418
Impact factor: 11.082
Abstract
A radiometric microbiologic assay has been developed for the determination of niacin in biologic fluids. Lactobacillus plantarum produced 14CO2 from L-[U-14C] hr malic acid in quantities proportional to the amount of niacin present. The assay is specific for the biologically active forms of niacin in humans. Thirty normal hemolysates were analyzed and the values ranged from 13.0 to 17.8 micrograms niacin/ml RBC (mean = 15.27 +/- 1.33 s.d.). Good recovery and reproducibility studies were obtained with this assay. On thirty blood samples, correlation was excellent between the radiometric and the conventional turbidimetric assays.
MeSH terms
Animals; Biological Assay; Body Weight; Carbon Dioxide; Carbon Radioisotopes; Lactobacillus; Malates; Nephelometry and Turbidimetry; Nicotinic Acids; Rats
More resources
EndNote: Download