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113426
Rickettsial hemolysis: rapid method for enumeration of metabolically active typhus rickettsiae.

J Clin Microbiol, 1979/5;9(5):645-7.

Walker TS, Winkler HH

PMID: 113426

Impact factor: 11.677

Abstract
A new assay is described for enumerating biologically active typhus rickettsiae (Madrid E strain), based on adsorption of rickettsiae to erythrocytes in the presence of NaF (which allows adsorption but not lysis) and lysis in the presence of anti-Rickettsia prowazeki immune serum (which allows only a single round of lysis). The number of lysed erythrocytes is then used to estimate the number of active rickettsiae.
MeSH terms
Animals; Bacteriological Techniques; Erythrocytes; Hemolysis; Rickettsia prowazekii; Sheep
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