A precursor of cytoplasmic actin in cultured Drosophila cells.
J Cell Biol, 1979/6;81(3):680-3.
PMID: 110818
Impact factor: 8.077
Abstract
In continuous lines of cultured Drosophila cells two forms of cytoplasmic actin, designated II and III, are detected after a 25- to 120-min pulse label with [35S]methionine. However, only one of these, actin II, accumulates in the cell. With the use of a pulse-chase protocol and two-dimensional gel electrophoresis, it has been found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated to be approximately 50 min.
MeSH terms
Actins; Animals; Autoradiography; Cells, Cultured; Cytoplasm; Drosophila melanogaster; Electrophoresis; Kinetics; Protein Precursors
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