A solid-phase radioimmunoassay for bacterial lipopolysaccharide.
Immunol Commun, 1979;8(3):347-64.
PMID: 108200
Abstract
A radioimmunoassay for E. coli 055:B5 lipopolysaccharide (LPS) is described. The LPS was derivatised by two new methods and subsequently radiolabeled with 125I to a specific activity of 2-4 mCi/mg without apparent loss in its biophysical, immunological or biological activities. Using antibody-coated polystyrene tubes, a solid-phase radioimmunoassay was developed with a sensitivity of 10-500 ng/ml of LPS.
MeSH terms
Animals; Antibodies, Bacterial; Chromatography, Gel; Escherichia coli; Immunodiffusion; Lethal Dose 50; Lipopolysaccharides; Lymphocyte Activation; Mice; Phenylacetates; Rabbits; Radioimmunoassay; Tyramine
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