Characterization and purification of a kallikrein from human pancreatic juice and immunological comparison with other kallikreins.
Hoppe Seylers Z Physiol Chem, 1979/3;360(3):457-65.
PMID: 108189
Abstract
A prekallikrein has been demonstrated in human pancreatic juice and the active enzyme has been purified from this material. The purification procedure included filtration on Sephadex G-100, chromatography on DEAE-cellulose and affinity chromatography on trypsin-inhibitor Sepharose. The purified kallikrein appeared to be homogeneous by polyacrylamide gel electrophoresis at pH 8.3 and by immunoelectrophoresis. Human pancreatic kallikrein is immunologically different from human plasma kallikrein and from pancreatic kallikreins of other species (hog, cat, rat and dog). Human pancreatic kallikrein has common antigenic determinants with human urinary and submandibular kallikreins but probably not with parotid kallikrein.
MeSH terms
Animals; Biological Assay; Blood Pressure; Cats; Cross Reactions; Dogs; Humans; Immunodiffusion; Immunoelectrophoresis; Kallikreins; Pancreatic Juice; Rats; Species Specificity; Swine
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