Thermodynamics of single mismatches in RNA duplexes.
Biochemistry, 1999/10/26;38(43):14214-23.
Kierzek R[1], Burkard ME, Turner DH
Affiliations
PMID: 10571995
Impact factor: 3.321
Abstract
The thermodynamic properties and structures of single mismatches in short RNA duplexes were studied in optical melting and imino proton NMR experiments. The free energy increments at 37 degrees C measured for non-GU single mismatches range from -2.6 to 1.7 kcal/mol. These increments depend on the identity of the mismatch, adjacent base pairs, and the position in the helix. UU and AA mismatches are more stable close to a helix end, but GG mismatch stability is essentially unaffected by the position in the helix. Approximations are suggested for predicting stabilities of single mismatches in short RNA duplexes.
MeSH terms
Adenine; Base Pair Mismatch; Base Pairing; Base Sequence; Guanine; Hot Temperature; Nuclear Magnetic Resonance, Biomolecular; Nucleic Acid Conformation; Nucleic Acid Heteroduplexes; RNA; RNA, Ribosomal; Thermodynamics; Uracil
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