Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives.
Mol Gen Genet, 1978/11/09;166(3):313-20.
Grinsted J, Bennett PM, Higginson S, Richmond MH
PMID: 105244
Abstract
The sites of insertion of Tn501 into RP1 and into derivatives of this plasmid that either lack the Tn801 (TnA) element or contain it in a different location have been determined. Similarly, the sites of insertion of Tn802 into a derivative of RP1 that lacks the Tn801 element and into recombinants of this plasmid with Tn501 were determined. 'Hot spots' for insertion were observed with both transposons; but it is clear that a particular DNA sequence is not sufficient to define a 'hot spot', since a particular region does contain many insertions when present in one plasmid but does not do so when part of another.
MeSH terms
Base Sequence; Chromosomes, Bacterial; DNA, Bacterial; Drug Resistance, Microbial; Escherichia coli; Genes; Pseudomonas aeruginosa; R Factors; Recombination, Genetic
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