Improved preparation and structural elucidation of the tryptophan cleavage reagent 2-(2'-nitro-phenylsulfenyl)-3-methyl-3-bromoindolenine (BNPS-skatole).
J Clin Chem Clin Biochem, 1978/12;16(12):669-74.
PMID: 105078
Abstract
Methods are described for a high yield preparation (overall yield greater than 90%) of analytically pure 2-(2'-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine (BNPS-skatole). The chromatographic and spectroscopic data are given. The yield of tryptophan cleavage is considerably dependent on the degree of purity of the BNPS-skatole.
MeSH terms
Chemical Phenomena; Chemistry; Humans; Immunoglobulin kappa-Chains; Indoles; Peptide Fragments; Proteins; Skatole; Sulfenic Acids; Tryptophan
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