Bacteriophage SPO1 development: defects in a gene 31 mutant.
J Virol, 1978/9;27(3):483-9.
PMID: 100606
Impact factor: 6.549
Abstract
SPO1 temperature-sensitive mutant ts14-1, located in cistron 31, has a DD (DNA synthesis-delayed) phenotype at 37 degrees C and produces progeny in a stretched program. At 44 degrees C it behaves as a DO (DNA synthesis-defective) mutant and shuts off the viral RNA synthesis about 10 min after infection. The thermal sensitivity of this mutant is due to the inactivity of gp-31 (the product of gene 31) at 44 degrees C. However, gp-31 is synthesized at that temperature and partly recovers its activity at 37 degrees C. Only 5 min at the permissive temperature is enough to trigger the continuation of the phage program and to produce progeny. The partial defect at 37 degrees C and the expansion of the middle program together with the pleiotropic defects at the nonpermissive temperature could be suitable for the study of the controls involved in bacteriophage development.
MeSH terms
Bacillus subtilis; Bacteriophages; Chloramphenicol; DNA, Viral; Genes, Viral; Mutation; Temperature; Transcription, Genetic; Virus Replication
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